What are the advantages of MEA to study in vitro epilepsy disease models?
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Epilepsy is a neurological disorder characterized by reoccuring seizures, often caused by abnormal electrical activity in the brain. Understanding the alterations in neural networks is crucial to unraveling the fundamental triggers and mechanisms behind epileptic seizures.
Axion’s live-cell assay platforms offer an ideal approach for investigating epilepsy and its effects on neural networks. Noninvasive, real-time analysis preserves the intricate connections and signaling within neural circuits, enabling researchers to observe and study the progression of epilepsy in vitro.
Understanding mechanisms of epilepsy and seizures with in vitro models
Studying epilepsy requires innovative lab tools designed to accurately measure complex neural network function.
The Maestro MEA platform can recreate in vivo phenotypes of seizurogenic activity in vitro, allowing researchers to:
- Compare patient lines side-by-side
- Screen therapeutics and assess functional impacts
- Monitor and evaluate cultures over time and label-free
Discover how Axion’s live-cell analysis tools can reveal the mechanisms of epilepsy.
Exploring neural activity with in vitro models of epilepsy and seizures
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Modulation of neuronal network activity to understand the mechanisms of epilepsy>
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Studying pediatric epilepsy with stem cell-based models>
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In vitro model to assess of the role of isocitrate dehydrogenase mutation in the development of glioblastoma-induced seizures>
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AI-based MEA assay assesses drug-induced neurotoxicity and seizure risk in vitro>
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Publication Highlights: Epilepsy>
Modulation of neuronal network activity to understand the mechanisms of epilepsy
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Purpose: To understand the effects of microRNA 128 on the excitability of neuronal networks. Recent studies have implicated the involvement of certain microRNAs in development of epilepsy.
The network excitability of primary mouse cortical neurons, with suppressed expression of microRNA 128, was studied on the Maestro MEA platform.
Result: The Maestro MEA data indicated that neurons with microRNA 128 knockdown expression exhibited increased overall firing, bursting, and number of network spikes, suggesting microRNA 128 suppression induces network hyperexcitability phenotype [McSweeney 2016].
Studying pediatric epilepsy with stem cell-based models
>
In vitro model to assess of the role of isocitrate dehydrogenase mutation in the development of glioblastoma-induced seizures
>
AI-based MEA assay assesses drug-induced neurotoxicity and seizure risk in vitro
>
Publication Highlights: Epilepsy
>
FAQ:
- The Maestro MEA platform offers a controlled environment for studying detailed neural network activity in vitro.
- High-throughput multiwell plates make it ideal for screening patient-specific lines and therapeutics.
- Noninvasive monitoring allows for the study of long-term effects and disease progression.
- It is easy to use, requiring only basic cell culturing techniques to measure neural electrophysiology.
What kind of neural cultures can be measured on the Maestro MEA?
The main requirement is that you have electrically active cells. Primary or stem cell-derived neurons can be used. They may be cocultured with our without glial cells. Neurons can be measured from 2D cultures, organoids or other 3D cultures, and slices.
What kind of metrics can you get from neural activity?
MEA measures from multiple areas of a culture over time, allowing you to go beyond just the firing of individual neurons and evaluate dynamic network activity and the development of functional phenotypes. Learn more about what you can do with our Neural Module.
