Drug Dosing Calculator Instructions
The Drug Dosing Calculator helps you design dose formulations for plate-based experiments. Enter your stock, target doses, and well setup, and the calculator will generate the intermediate and working solutions needed to dose the plate.
Step 1: Starting Stock Conditions
Enter the concentration of your compound stock and solvent (vehicle) it is dissolved in. For example, a compound prepared in all DMSO would typically be entered as 100% DMSO.
Step 2: Target Dose Conditions
Enter the final dose concentrations you want in the wells.
Each row represents one dose group. For each group, enter the target dose, select the units, and enter the number of replicates.
Use + Add Dose Row to add more dose groups, or the remove button to delete a row.
Enter a Spare Volume % to prepare extra solution for pipetting loss or dead volume.
Step 3: Experiment Setup
Enter the well conditions for the experiment: the Starting Well Volume, the desired final vehicle concentration in the well, such as 0.1% DMSO, the Dose Volume, and the addition method.
This calculator is provided as a planning aid for research use only. Results should be independently reviewed and verified before use in an experiment. Axion BioSystems is not responsible for errors resulting from incorrect inputs, assumptions, or calculations.
