Authors: Alexander Sonntag, Hardy Mitdank and Alexander Weng
Gene Therapy of Cancer, 23 June 2022
Suicide gene therapy is a therapeutic strategy based on the delivery of suicide genes to cancer cells, which then kill themselves by expressing toxic ribosome-inactivating proteins. Plasmid DNA vectors are easy-to-produce, but are known to cause immunological side effects. To counteract this, the minicircle technology can be used. The authors of this article describe a methodology to produce minicircle-DNA at low costs. Following transfection, the cell killing properties were monitored with the Axion BioSystems Omni and analyzed using the Axion BioSystems Confluency Module.
