Download Authors: Hongbo Yuan, Kaizheng Liu, Melissa J. J. van Velthoven, Jyoti Kumari, Yuying Bao, Susana Rocha, and Paul H. J. KouwerNature Protocols, 30 May 2025 Related Resources Live-cell Imaging with 3D Cell Models Real-time analysis of 3D cell culture models using live-cell imaging Novel methods for assessing electrophysiological function of cardiac and neural 3D cell cultures with MEA technology Related Products Image Lux