Cell culture protocol for recording from CDI iCell® DopaNeurons on Maestro MEA systems
Abstract:
iCell® DopaNeurons, human induced pluripotent stem cell (iPSC)-derived neurons, exhibit biochemical, electrophysiological, and pathophysiological properties characteristic of native human neurons. Due to their high purity, functional relevance, and ease of use, iCell DopaNeurons represent an optimal in vitro test system for neurobiology interrogations in basic research and many areas of drug development. Axion BioSystems’ Maestro multielectrode array (MEA) technology is a noninvasive, label-free platform that measures the electrical activity of single cells or cellular networks. With proper handling, iCell DopaNeurons can be thawed and cultured directly on MEAs to form neuronal networks amenable to electrophysiological interrogation. This Application Protocol describes how to thaw, plate, and culture iCell DopaNeurons on the Maestro MEA system and provides basic instructions for data acquisition and analysis.
