Lumos - Brochure

Fast, flexible set-up

AxIS Navigator provides intuitive, visual stimulation programming in an easily accessible plate map format. Here, each of the LEDs can be independently controlled for both intensity (1-100% max power) and duration (down to 100 microseconds). Additionally, stimulation across the plate can be set up for predefined time courses or regulated via an operator start-stop interface in the software.

The Lumos system can be used in two general ways

Control of cell biology – Deliver light to Axion’s Lumos OptiClear plates (24- and 48-well formats), standard-format SLAS-compatible microplates with optical specializations and clear bottom.

Control of cell activity – Pair Lumos with Axion’s Maestro microelectrode array (MEA) platform and deliver light to Axion’s Lumos MEA plates for measurement of network activity.

Control of cellular biology on a high-throughput scale

Light-mediated control of biochemical, genetic, and cellular functions is an emerging tool in many research fields:

Control intracellular signaling pathways – e.g., Kennedy et al. 2010
Direct gene expression with light – e.g., Konermann et al. 2013
Edit genes with light-induced CRISPR/Cas9 – e.g., Polstein & Gersbach 2015

Perform extended light delivery experiments on the bench

Temperature – Compatible with standard lab warming plates
CO₂ – Gas delivery port enables a controlled CO₂ environment
Humidity – Lumos plate humidity reservoirs reduce evaporation from wells

Optimal light delivery and imaging

Lumos OptiClear 24 (OPT-24) and 48 (OPT-48) are Axion’s first microplates without electrodes. These plates have a high clarity bottom culture surface for microscopy and high content imaging. The specially formulated reflective white walls and lensed lid facilitate optimal light delivery within each well.

Decode life’s circuitry with MEA

The Maestro systems are the industry’s first high-throughput microelectrode array (MEA) systems. Record networks of electrically active cells (e.g., neurons, cardiomyocytes, and muscle cells) in a multiwell plate in real time. Over minutes or months, gain unprecedented access to electrical network function from cultured cell populations. Perfect for characterizing newly created iPS cells, researching the behavior of diseased cells, screening for drugs to treat that disease, or examining potential toxic effects of drug candidates, the Pro and Edge systems deliver an easy-to-use, compact cellular analysis system suitable for any lab.

Designed for optogenetic MEA studies

Lumos MEA 24 (M384-tMEA-24OPT) and 48 plates (M768-tMEA-48OPT) for the Maestro Pro and Edge MEA platforms combine unparalleled access to cellular electrical network information (16 recording electrodes per well) with a thin, transparent plate bottom for culture visualization and assay multiplexing. The specially formulated reflective white walls and lensed lid facilitate optimal light delivery within each well.

Control cell activity with light

Optogenetics techniques use genetic targeting to express light-sensitive ion channels called opsins (e.g., ChR2) in targeted cell populations. Upon stimulation by a specific wavelength of light, these opsins can be activated to shift the cell membrane potential. Light of specific wavelengths can thus be used to activate or silence cell activity in targeted cell populations.

Advantages of optogenetic-mediated control of cell activity include:
Artifact-free stimulation – Light-evoked electroactivity is artifact-free
Precise control of cell activity – Study a range of physiologically relevant states in each well
Reduced experimental variability – Standardize cell activity to reduce variability in well-to-well and batch-to-batch activity
Cell specific control – Enhanced neural disease modeling is possible with cell type specific control in mixed cell cultures

The most flexible in vitro optical stimulation system

A first-of-its-kind on the market, Lumos revolutionizes in vitro optical approaches. Lumos provides 24 or 48 concurrent wells of stimulation and the ability to simultaneously use up to four different wavelengths of light per well at user-specified, precisely defined output patterns.

(Left) Application of FPL 64176 induced a significant prolongation of BP and FPD relative to the vehicle control (DMSO). (Right) Pacing at 700 ms BP controls for the influence of beating rate on repolarization.

Under paced conditions, FPL-64176 produced a dose-dependent prolongation of FPD that was independent of beat period.